Usha P. Andley, PhD

Professor, Ophthalmology and Visual Sciences

Office Phone: (314) 362-5891

Email: Andley@vision.wustl.edu

Click here for Dr. Andley lab website

My laboratory studies the biochemical basis of human cataract formation. Several approaches are being used at understanding the function of alpha-crystallin, a major protein of the lens and a member of the small heat shock protein family of molecular chaperones. This protein, composed of two gene products alpha A and alpha B, plays important roles in the lens and other tissues. Mutations in alpha A and alpha B crystallins form the basis of several hereditary cataracts. To understand the function of alpha-crystallin in the lens, and how deterioration of chaperone function results in cataract development., we are using several genetic and biochemical approaches.

Education

  • B.Sc. Delhi University, India (Chemistry Honors), 1970
  • M.Sc., Physical Chemistry, Delhi University, India, 1972
  • Ph.D., Biochemistry, Jawaharlal Nehru University, India, 1977

Training

  • Research Fellow, Berman-Gund Laboratory for the Study of Retinal Degenerations, Harvard Medical School, Massachusetts Eye and Ear Infirmary, Boston, MA, 1977-1979
  • Research Associate, Eye Research Institute of the Retina Foundation, Boston, MA, 1979-1982
  • Molecular Biology and Biotechnology Workshop, Smith College, Northampton, MA, 1991

Honors and Awards

  • Chemistry Honors First Prize Award, Miranda House, Delhi University, India, 1969 & 1970
  • Full Scholarship, University Grants Commission, New Delhi, India, 1972-1977
  • Charles A. King Trust Fellowship, The Medical Foundation, Boston, MA, 1982-1983
  • Cooperative Cataract Research Group Travel Award, 1989
  • Research to Prevent Blindness Robert E. McCormick Research Scholar Award, 1996
  • Research to Prevent Blindness Lew R. Wasserman Award, 2000
  • National Foundation for Eye Research Award for Cataract Research, 2004
  • Fellow, Association for Research in Vision and Ophthalmology, 2010

Selected Publications

  1. Andley UP, Goldman JW. Autophagy and UPR in alpha-crystallin mutant knock-in mouse models of hereditary cataracts. Biochim Biophys Acta. 2016;1860(1 Pt B):234-9.
  2. Makley LN, McMenimen KA, DeVree BT, Goldman JW, McGlasson BN, Rajagopal P, et al. Pharmacological chaperone for α-crystallin partially restores transparency in cataract models. Science. 2015;350(6261):674-7.
  3. Andley UP, Malone JP, Townsend RR. In vivo substrates of the lens molecular chaperones alphaA-crystallin and alphaB-crystallin. PLoS One. 2014;9(4):e95507.
  4. Andley UP, Malone JP, Hamilton PD, Ravi N, Townsend RR. Comparative proteomic analysis identifies age-dependent increases in the abundance of specific proteins after deletion of the small heat shock proteins alphaA- and alphaB-crystallin. Biochemistry. 2013;52(17):2933-48.
  5. Sharma A, Pirouzmanesh A, Patil J, Estrago-Franco MF, Zacharias LC, Pirouzmanesh A, et al. Evaluation of the toxicity of triamcinolone acetonide and dexamethasone sodium phosphate on human lens epithelial cells (HLE B-3). J Ocul Pharmacol Ther. 2011;27(3):265-71.
  6. Ehrenshaft M, Zhao B, Andley UP, Mason RP, Roberts JE. Immunological detection of N-formylkynurenine in porphyrin-mediated photooxided lens alpha-crystallin. Photochem Photobiol. 2011;87(6):1321-9.
  7. Andley UP, Malone JP, Townsend RR. Inhibition of lens photodamage by UV-absorbing contact lenses. Invest Ophthalmol Vis Sci. 2011;52(11):8330-41.
  8. Andley UP, Hamilton PD, Ravi N, Weihl CC. A knock-in mouse model for the R120G mutation of alphaB-crystallin recapitulates human hereditary myopathy and cataracts. PLoS One. 2011;6(3):e17671.
  9. Zhao B, Chignell CF, Rammal M, Smith F, Hamilton MG, Andley UP, et al. Detection and prevention of ocular phototoxicity of ciprofloxacin and other fluoroquinolone antibiotics. Photochem Photobiol. 2010;86(4):798-805.
  10. Watson GW, Andley UP. Activation of the unfolded protein response by a cataract-associated alphaA-crystallin mutation. Biochem Biophys Res Commun. 2010;401(2):192-6.

Click to view all Dr. Andley Publications

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