Bassnett Lab / CV & Publications

Curriculum Vitae — Steven Bassnett

Address:	Washington University School of Medicine Department of Ophthalmology and Visual Sciences 660 S. Euclid Ave. Campus Box 8096 St. Louis, MO 63110 (314) 362-1604 office (314) 362-1650 lab (314) 362-3638 fax bassnett@vision.wustl.edu
Present Position:	Professor Department of Ophthalmology and Visual Sciences Department of Cell Biology and Physiology Washington University School of Medicine St. Louis, MO
Education:
Undergraduate: 1979-1982	B.Sc. Marine Biology, School of Animal Sciences University College of North Wales, Bangor, UK
Graduate: 1982 - 1987	Ph.D. Biophysics, School of Biological Sciences University of East Anglia (UEA), UK
Postgraduate
1987	Postdoctoral Fellow in Biophysics, UEA (Laboratory of Dr. George Duncan)
1987 - 1990	Postdoctoral Fellow, Department of Anatomy and Cell Biology, Uniformed Services University, Bethesda, MD (Laboratory of Dr. David Beebe)
Academic Positions / Employment:
1990 - 1995	Research Assistant Professor Department of Anatomy and Cell Biology Uniformed Services University Besthesda, MD
1995 - 2001	Assistant Professor Department of Ophthalmology and Visual Sciences Department of Cell Biology and Physiology Washington University School of Medicine St. Louis, MO
2001 - 2007	Associate Professor Department of Ophthalmology and Visual Sciences Department of Cell Biology and Physiology Washington University School of Medicine St. Louis, MO
2007 - present	Professor Department of Ophthalmology and Visual Sciences Department of Cell Biology and Physiology Washington University School of Medicine St. Louis, MO
University / Departmental Appointments and Committees
Departmental Responsibilities:
1996 - 2006	Designed, implemented and maintained Departmental website: http://ophthalmology.wustl.edu
1998	Local co-organizer of VISION, an NIH-sponsored traveling exhibit shown at the St. Louis Science Center
1999	Special Senses lecture for minority high school students St. Louis Academy of Sciences
2000 - present	Designed, implemented and maintain Vision Research Intranet
1999 - present	Digital Imaging Module Director for Vision Science Core Grant (ET02687)
2006 - present	Histology Module Director for Vision Science Core Grant (ET02687)
2000 - present	Resident Education Committee, Space Committee, Equipment Committee
2001	Departmental Representative Washington University Health Professions Fair (minority student recruiting event)
University Responsibilities:
2001 - 2006	Thesis advisor: Anna Zandy
2001 - 2004	Thesis advisor: Richard McNulty
1998 - 2003	Thesis committee: Brian Cobb
2004 - 2007	Thesis committee: Ramya Rajagopal
2004 - present	Thesis committee: Kelly Barton
Editorial Responsibilities:
Editorial Board 2000 - present	Experimental Eye Research
Guest Editor 2000	Investigative Ophthalmology and Visual Sciences
Ad hoc Reviewer	Nature; American Journal of Physiology; Ophthalmic Research; Experimental Eye Research, Current Eye Research; Investigative Ophthalmology and Visual Sciences; Journal of Histochemistry and Cytochemistry; Journal of Physiology (Lond.), Applied Biomaterials, Seminars in Cell and Developmental Biology.
Peer Review Responsibilities:
2004 - 2008	NIH AED (Anterior Eye Disease) Study Section (Chair from 2006-2008)
Grant Review:
2003	Lens and Cataract Panel: National Eye Institute strategic plan
1997	Ad hoc reviewer for NIH VISA study section
1998	Veterans Administration Merit Review Board
2000 - 2003	Fight for Sight Grants in Aid
2001	Ad hoc reviewer for NIH NRSA program
1998 - 2002	American Federation for Aging Research (AFAR)
Professional Societies and Organizations:
	Association for Research in Vision and Ophthalmology International Society for Eye Research
Research Support:
Government:
1992 - 1996	Fiber Cell Formation in Normal and Cataractous Lenses R01 EY09852 Principal Investigator; Total Direct Costs $ 328,000
1996 - 2001	Fiber Cell Formation in Normal and Cataractous Lenses R01 EY09852 Principal Investigator; Total Direct Costs $ 645,000
2001 - 2007	Fiber Cell Formation in Normal and Cataractous Lenses R01 EY09852 Principal Investigator; Total Direct Costs $ 1,000,000
2007 - 2011	Fiber Cell Formation in Normal and Cataractous Lenses. R01 EY09852 Principal Investigator; Total Direct Costs $ 1,125,000
2002 - 2003	Supplement to RO1 EY09852 for provision of confocal microscope facility: $400,000
1999 - 2004	Functional Organization of Membrane Domains in the Lens R01 EY12260 Principal Investigator; Total Direct Costs $ 870,000
2004 - 2007	Oxygen Metabolism and Cataract R01 Grant. Total Direct Costs $ 1,125,000
1995 - 1998	Lens Major Intrinsic Protein in Hereditary Cataract R01 EY11411 Co-Principal Investigator; Total Direct Costs $ 482,000 (with Dr. Alan Shiels)
2008 - 2012	Lim2 and the Lens Core Syncytium. Principal Investigator; Total Direct Costs $ 1,250,000
2005 - 2010	Core Grant For Vision Research. 5P30EY002687-30 Principal Investigator
Non-Government:
1990 - 1992	NATO collaborative grant Co-Principal Investigator; Total Direct Costs $7,000 (with Dr. Tim Jacob, University of Cardiff, UK)
1997 - 2001	RPB Career Development Award Principal Investigator; Total Direct Costs $160,000
2002	RPB William and Mary Greve Scholar Award
2008	RPB Wasserman Award $60,000
Teaching Responsibilities
Resident Research:
1997, 1999	Resident Research Project Director (Drs. Druger and Young)
Lecturer:
1996	Biological Applications of Optical Microscopy (Bio 5662)
1999 - present	Lecture: Physiology of the Eye (Ophthalmology Residents)
2000	Lecturer: Ethics and Research Science (Bio 5011)
2005 - present	Lecturer: Biology and Pathology of the Visual System
Postdoctoral Fellows:
1996 - 1998	Dr. Danijela Mataic, M.D. (currently in private practice, St. Louis)
1998 - 1999	Dr. Ivica Vucemilo, M.D. (currently surgery resident, McMaster University, Canada)
1999 - 2000	Dr. Dan Eisenberg M.D. (currently surgery resident, Yale University)
1998 - 2003	Dr. Valery Shestopalov, Ph.D. (currently Assistant Professor of Ophthalmology, Bascom Palmer Eye Institute, Miami)
1999 - 2002	Dr. Beverly Faulkner-Jones, M.D., Ph.D. (currently Assistant Professor of Pathology, University of New Mexico)
2002	Dr. Wuquas Ilyas, M.D. (currently ophthalmology resident, U. VA)
2004 - present	Dr. Alicia deMaria
2004 - present	Dr. Yanrong Shi
2004 - 2007	Dr. Xianmin Luo (Staff Scientist, Department of Cell Biology, Washington University, St. Louis)

Bibliography

Peer-Reviewed Manuscripts:

1. Bassnett S, Duncan G: Direct Measurement of pH in the rat lens by ion-sensitive microelectrodes. Experimental Eye Research 1985; 40:585-590.
2. Bassnett S, Croghan PC, Duncan G: Diffusion of lactate and its role in determining intracellular pH in the lens of the eye. Experimental Eye Research.1987;44:143-147.
3. Bassnett S. Ion activity and membrane function in the lens: Ph.D. thesis 1987. University of East Anglia, UK.
4. Lucas VA, Bassnett S, Duncan G, Stewart S, Croghan PC: Membrane conductance and potassium permeability of the rat lens. Quarterly Journal of Experimental Physiology 1987; 72:81-93.
5. Bassnett S, Duncan G: The influence of pH on membrane conductance and intercellular resistance in the rat lens. Journal of Physiology 1988; 398:507-521.
6. Bassnett S, Stewart S, Duncan G, Croghan PC: Efflux of chloride from the rat lens: influence of membrane potential and intracellular acidification. Quarterly Journal of Experimental Physiology 1988; 73:941-949.
7. Bassnett S, Reinisch L, Beebe DC: Intracellular pH measurement using single excitation - dual emission fluorescence ratios. American Journal of Physiology 1990;258:C171-C178.
8. Bassnett S, Beebe DC: Localization of insulin-like growth factor-1 binding sites in the embryonic chicken eye. Investigative Ophthalmology and Visual Science 1990; 31:1637-1643.
9. Bassnett, S: Intracellular pH regulation in the embryonic chicken lens epithelium. Journal of Physiology (Lond.). 1990; 431:445-464.
10. Bassnett S, Becker TE, Beebe DC: Ion concentrations, fluxes and electrical properties of the isolated embryonic chicken lens. Experimental Eye Research 1992; 54:215-224.
11. Bassnett S, Beebe DC: Coincident loss of mitochondria and nuclei during lens fiber cell differentiation. Developmental Dynamics. 1992;195:85-93.
12. Bassnett S: Mitochondrial dynamics in differentiating fiber cells of the mammalian lens. Current Eye Research 1992; 11:1227-1232.
13. Bassnett S, Kuszak JR, Reinisch L, Brown HG, Beebe DC: Intercellular communication between epithelial and fiber cells of the eye lens. Journal of Cell Science 1994; 107:799-811.
14. Potts JD, Bassnett S, Kornacker S, Beebe DC: Expression of platelet-derived growth factor receptors in the developing chicken lens. Investigative Ophthalmology and Visual Sciences 1994; 35:3413-3421.
15. Gao CY, Bassnett S, Zelenka PS. Cyclin B, p34cdc2, and H1-kinase activity in terminally differentiating lens fiber cells: Developmental Biology 1995; 169:185-194.
16. Potts JD, Bassnett S, Beebe DC. Expression of transforming growth factor in the embryonic avian lens coincides with the presence of mitochondria. Developmental Dynamics: 1995;203:317-323.
17. Bassnett S. The fate of the Golgi apparatus and the endoplasmic reticulum during lens fiber cell differentiation. Investigative Ophthalmology and Visual Sciences: 1995; 36:1793-1803.
18. Shiels A, Bassnett S. Mutations in the founder of the MIP gene family underlie cataract development in the mouse. Nature Genetics: 1996; 12:212-215.
19. Bassnett S, Mataic D. Chromatin degradation in differentiating fiber cells of the eye lens. Journal of Cell Biology: 1997:137:37-49.
20. Bassnett S. Fiber cell denucleation in the primate lens. Investigative Ophthalmology and Visual Science: 1997; 38:1678-1687.
21. Li X, Cvekl A, Bassnett S, Piatigorsky J. Multiple regulatory sequences and transcription factors regulate differential expression of avian δ-crystallin/arginosuccinate lyase genes in the lens. Devopmental Genetics: 1997; 20:258-266.
22. Andley UP, Song Z, Wawrousek EF, Bassnett S. The molecular chaperone αA crystallin enhances lens epithelial cell growth and resistance to UVA stress. Journal of Biological Chemistry:1998; 273:31252-31261.
23. Bassnett S, Vucemilo I, Missey H. Molecular architecture of the lens fiber cell basal adhesion complex. Journal of Cell Science: 1999; 112:2155-2165.
24. Shestopalov VI, Bassnett S. Exogenous gene expression and protein targeting in lens fiber cells. Investigative Ophthalmology and Visual Science: 1999; 40:1435-1443.
25. Berthoud VM, Bassnett S, Beyer EC. Cultured chicken embryo lens cells resemble differentiating fiber cells in vivo and contain two kinetic pools of connexin56. Experimental Eye Research: 1999; 68:475-484.
26. Varadaraj K, Kushmerick C, Baldo GJ, Bassnett S, Shiels A, Mathias RT. Role of MIP in lens fiber cell membrane transport. Journal of Membrane Biology: 1999;170:191-203.
27. Shestopalov VI, Bassnett S. Three dimensional organization of primary lens fiber cells. Investigative Ophthalmology and Visual Sciences: 2000; 41:1-5.
28. Cho K-S, Joo C-K, Williams J, Ambroziak B, Bassnett S, Pepose JS, Fleming TP. Stimulation of quiescent corneal endothelial cells by direct delivery of the SV40 large T-antigen protein. Current Eye Research: 2000; 20:58-63.
29. Shiels A, Mackay D, Bassnett S, Al-Ghoul K, Kuszak JR. Disruption of lens fiber cell architecture in mice expressing a chimeric AQP0-LTR protein. FASEB Journal: 2000; 14:2207-2212.
30. Shestopalov VI, Bassnett S. Expression of autofluorescent proteins reveals a novel protein permeable pathway between cells in the lens core. Journal of Cell Science: 2000; 113:1913-1921.
31. Andley UP, Song Z, Wawrousek E, Bassnett S, Fleming TP. Lens epithelial cells derived from αB-crystallin knockout mice demonstrate hyperproliferation and genomic instability. FASEB Journal: 2001; 15:221-229.
32. Andley UP, Song Z, Wawrousek E, Fleming TP, Bassnett S. Differential protective activity of αA- and αB-crystallin in lens epithelial cells. Journal of Biological Chemistry: 2000; 275:36823-36831.
33. Beebe DC, Vasiliev V, Guo J, Shui Y-B, Bassnett S. Changes in adhesion complexes define stages in the differentiation of lens fiber cells. Investigative Ophthalmology and Visual Science: 2001; 42:727-34.
34. Shestopalov VI, Missey H, Bassnett S. Delivery of genes and fluorescent dyes into cells of the intact lens by particle bombardment. Experimental Eye Research: 2002; 74:639-649.
35. Faulkner-Jones B, Zandy A, Bassnett S. Differential RNA stability during lens fiber cell maturation. Experimental Eye Research: 2003; 77:463-476.
36. Shiels A, Bassnett S, Varadaraj K, Mathias R, Al-Ghoul K, Kuszak J, Donoviel D, Lilleberg S, Friedrich G, Zambrowicz B. Optical dysfunction of the crystalline lens in aquaporin-0-deficient mice. Physiological Genomics. 2001;7:179-186.
37. Bassnett S. Lens organelle degradation. Experimental Eye Research. 2002;74:1-6.
38. Bassnett S, Winzenburger, PA. Morphometric analysis of fiber cell growth in the developing chicken lens. Experimental Eye Research. 2003; 76:291-302.
39. Hennig AK, Galvin N, Mosinger Ogilvie J, Vogler CA, Levy B, Bassnett S, Sands MS. Intra-vitreal gene therapy reduces lysosomal storage in the brains of mucopolysaccharidosis VII mice. Journal of Neuroscience 2003; 23:3302-3307.
40. Shestopalov VI, Bassnett S. Development of a macromolecular diffusion pathway in the lens. Journal of Cell Science 2003; 116:4191-4199.
41. Bassnett S, McNulty R. The effect of elevated intraocular oxygen on organelle degradation in the embryonic chicken lens. Journal of Experimental Biology 2003; 206:4353-4361.
42. McNulty R, Wang H, Mathias RT, Ortwerth BJ, Truscott RW, Bassnett S. Regulation of tissue oxygen levels in the mammalian lens. Journal of Physiology 2004; 559:883-898.
43. Zandy AJ, Lakhani S, Zheng T, Flavell RA, Bassnett S. Role of executioner caspases during lens development. Journal of Biological Chemistry 2005; 280:30263-72.
44. Bassnett S. Three-dimensional reconstruction of cells in the living lens: The relationship between cell length and volume. Experimental Eye Research 2005; 81:716-23.
45. Zandy AJ, Bassnett S. Proteolytic mechanisms underlying organelle degradation in the ocular lens. In review, Investigative Ophthalmology and Visual Sciences.
46. Lens fluorescence and accommodative amplitude in presbyopia and prepresbyopic subjects. Luo X, Kymes S, Gordon M, Bassnett S. Submitted to Investigative Ophthalmology and Visual Sciences.
47. Shiels A, Bassnett S. Refractive anomalies in Lim2-null mice. In review, Investigative Ophthalmology and Visual Sciences.
48. De Maria, Bassnett S. Role of DNAse IIB in lens fiber denucleation. In preparation.

Book chapters and Invited Publications

1. Bassnett S, Duncan G. Variation of pH with depth in the rat lens measured by double-barreled ion sensitive microelectrodes. In The Lens: Transparency and Cataract: Proceedings of the EURAGE/BBS symposium (Duncan G. Ed.), Rijswijk, Netherlands, Eurage, 1986; pp 77-85.
2. Bassnett S Ion activity and membrane function in the lens: Ph.D. thesis 1987. University of East Anglia, UK.
3. Berthoud VM, Westphale EM, Kurata WE, Bassnett S, Lau AF, Lampe DL, Beyer EC. Phosphorylation of chicken Cx56: possible implications. In: Gap Junctions (Werner R., ed.). IOS Press, Amsterdam, The Netherlands; 1997:205-209.
4. Bassnett S, Beebe DC. Fiber cell differentiation. In: Development of the Ocular Lens (Lovicu FJ and Robinson ML., eds.) Cambridge University Press, Cambridge, UK; 2004.
5. Bassnett S. The Eyes Have It. Review of Development of the Vertebrate Eye (Fini ME., Ed). Journal of Cell Science 2001; 114:5-8.
6. McNulty R, Bassnett S. Oxygen and the ocular lens: a key to understanding nuclear cataract? Physiology News, Cambridge, UK; 2005.