Bassnett Lab / Methods

Gene Delivery Techniques for the Lens

• Lens Vesicle Injection
• Transfection
• Intracellular Microinjection
• Particle Bombardment

Microscopy

• Paraformaldehyde preparation/Fixation/Vibratome slicing
• Immunofluorescence on lens slices
• Phalloidin staining of f-actin
• Staining nuclear DNA with Propidium Iodine

Molecular Techniques

• Tail DNA Extraction
• Sequencing Protocol
• In Situ Hybridization on lens slices

The Embryonic Lens

• wet weight and protein content size