Petrash Lab / CV & Publications

Curriculum Vitae - J. Mark Petrash

Address:	Washington University School of Medicine Department of Ophthalmology and Visual Sciences 660 S. Euclid Ave. Campus Box 8096 St. Louis, MO 63110 (314) 362-3335 office (314) 362-1171 laboratory (314) 362-3638 FAX petrash@wustl.edu
Present Position:	Professor Department of Ophthalmology and Visual Sciences Department of Genetics Washington University School of Medicine
Education:
1974-1977	B.S. Zoology, University of Texas, Austin, TX
1977-1981	Ph.D. Human Biological Chemistry and Genetics University of Texas Medical Branch, Galveston. Supervisor: Satish K. Srivastava, Ph.D.
1981-1984	Postdoctoral Research Fellow, Department of Biochemistry New York University, New York, NY Supervisor: John H. Chen, Ph.D.
Academic Positions:
1984-1988	Research Assistant Professor of Ophthalmology Emory University School of Medicine Atlanta, Georgia
1988-1993	Assistant Professor of Ophthalmology and Visual Sciences Assistant Professor of Genetics Washington University School of Medicine St. Louis, MO
1993-2000	Associate Professor of Ophthalmology and Visual Sciences Associate Professor of Genetics Washington University School of Medicine St. Louis, MO
2000-present	Professor of Ophthalmology and Visual Sciences Professor of Genetics Washington University School of Medicine St. Louis, MO
1991-present	Director of Research Department of Ophthalmology and Visual Sciences Washington University School of Medicine
Honors and Awards:
1988	Robert E. McCormick Research Scholar Award Research to Prevent Blindness, Inc.
1997	Lew R. Wasserman Merit Award Research to Prevent Blindness, Inc.
2000	Outstanding Faculty Mentor Award Graduate Student Senate of Arts & Sciences Washington University in St. Louis
Editorial Activities:
Frequent reviewer for Biochemistry, Biochem et Biophysica Acta, Current Eye Research, Diabetes, Experimental Eye Research, European Journal of Biochemistry, J Biological Chemistry, Molecular Vision
1995-present	Editorial Board: Molecular Vision (www.molvis.org/molvis/)
Consulting/Advisory Experience:
1993-1995	Fight for Sight Grant Review Panel
1999-2004	NIH/Center for Scientific Review permanent appointment to AED1 (former VISA1) study section; Chair 2002-2004
Professional Societies and Organizations:
American Association for Advancement of Sciences American Chemical Society American Diabetes Association American Society for Biochemistry and Molecular Biology Association for Research in Vision and Ophthalmology
Teaching Title and Responsibilities:
1992-present	"Nucleic Acids and Protein Synthesis"; Bio 548 Discussion Leader
1990	Coursemaster, "Molecular Aspects of Vision", Bio 5503
1989-1994	Thesis advisor to Ivan Tarle, MSTP student.
1996-2001	Thesis advisor to Brian Cobb, student in Biochemistry Program of DBBS
2001-present	Thesis advisor to Kelly Barton, student in Biochemistry Program of DBBS

Selected Publications:

Barton KA, Shui Y-B, Petrash JM, Beebe DC: Comment on: “The Stokes-Einstein equation and the physiological effects of vitreous surgery.” Acta Ophthal Scand (in press, January 2007)

Petrash JM, Harter TM, Devine C, Olins P, Bhatnagar A, Liu SQ, Srivastava SK: Involvement of cysteine residues in catalysis and inhibition of human aldose reductase: Mutagenesis of Cys-80, -298 and -303. J Biol Chem 267:24833-24840, 1992.

Borhani DW, Harter TM, Petrash JM: The crystal structure of the aldose reductase.NADPH binary complex. J Biol Chem 267: 24841-24847, 1992.

Tilton RG, Chang K, Hasan KS, Smith S, Petrash JM, Misko TP, Moore WM, Currie MG, Corbett JA, McDaniel ML, and Williamson JR. Prevention of diabetic vascular dysfunction by aminoguanidine and methylguanidine: Inhibition of nitric oxide synthase versus inhibition of advanced glycation endproduct formation. Diabetes 42:221-232, 1993.

Wilson DK, Tarle I, Petrash JM, Quiocho FA. Refined 1.8Å structure of human aldose reductase complexed with the potent inhibitor zopolrestat. Proc Natl Acad Sci USA 90:9847-9851, 1993.

Tarle I, Borhani DW, Wilson DK, Quiocho FA, Petrash JM. Probing the active site of human aldose reductase: Site-directed mutagenesis of Asp-43, Tyr-48, Lys-77 and His-110. J Biol Chem 268:25687-25693, 1993.

Hay RE, Andley UP, Petrash JM. Expression of recombinant bovine gB-, gC- and gD-crystallins and correlation with native proteins. Exp Eye Res 58:573-584, 1994.

Petrash JM, Tarle I, Wilson DK, Quiocho FA: Aldose reductase catalysis and crystallography: Insights from recent advances in enzyme structure and function. Diabetes 43:955-959, 1994.

Wilson DK, Nakano T, Petrash JM, Quiocho FA. 1.7 Å Structure of FR-1, a fibroblast growth factor-induced member of the aldo-keto reductase family, complexed with coenzyme and inhibitor. Biochemistry 34:14323-14330, 1995.

Das KP, Petrash JM and Surewicz WK. Conformational properties of substrate proteins bound to a molecular chaperone a- crystallin. J Biol Chem 271:10449-10452, 1996.

Nakano T and Petrash JM. Kinetic and spectroscopic evidence for active site inhibition of human aldose reductase. Biochemistry 35:11196-11202, 1996.

Andley UP, Mathur S, Griest TA and Petrash JM. Cloning, expression and chaperone-like activity of human aA crystallin. J Biol Chem 271:31973-31980, 1996

Cappiello M, Voltarelli M, Cecconi I, Vilardo PG, Dal Monte M, Marini I, Del Corso A, Wilson DK, Quiocho FA, Petrash JM and Mura U. Specifically targeted modification of human aldose reductase by physiological disulfides. J Biol Chem 271:33539-33544, 1996

Chandra A, Srivastava S, Petrash JM, Bhatnagar A, Srivastava SK. Modification of aldose reductase by S-nitrosoglutathione. Biochemistry 36:15801-15809, 1997

El-Kabbani O, Wilson DK, Petrash JM, Quiocho FA: Structural features of the aldose reductase and aldehyde reductase inhibitor-binding sites. Molec Vision 4:19 (http://molvis.org/molvis/v4/p19/), 1998

Srivastava S, Harter TM, Chandra M, Bhatnagar A, Srivastava SK, Petrash JM: Kinetic studies of FR-1, a growth factor-inducible aldo-keto reductase. Biochemistry 37:12909-12917, 1998

Cecconi I, Moroni M, Vilardo PG, Dal Monte M, Borella P, Rastelli G, Costantino L, Garland D, Carper D, Petrash JM, Del Corso A, Mura U: Oxidative modification of bovine lens aldose reductase induced by copper ion. Biochemistry 37:14167-14174, 1998

Srivastava K, Watowich SJ, Petrash JM, Srivastava SK, Bhatnagar A: Structural and kinetic determinants of aldehyde reduction by aldose reductase. Biochemistry 38:42-54, 1999

Das KP, Choo-Smith LP, Petrash JM, Surewicz WK: Insight into the secondary structure of non-native proteins bound to a molecular chaperone a-crystallin: An isotope-edited infrared spectroscopic study. J Biol Chem 274:33209-33212, 1999

Reddy GB, Das KP, Petrash JM, Surewicz WK: Temperature-dependent chaperone activity and structural properties of human aA and aB crystallins. J Biol Chem 275: 4565-4570, 2000

Cobb BA and Petrash JM: Characterization of a-crystallin-plasma membrane binding. J Biol Chem 275: 6664-6672, 2000

Dixit B, Balendiran GK, Watowich SJ, Srivastava S, Ramana KV, Petrash JM, Bhatnagar A, Srivastava SK: Kinetic and structural characterization of the glutathione binding site of aldose reductase. J Biol Chem 275:21587-21595, 2000

Cobb BA and Petrash JM: Structural and functional changes in the aA crystallin R116C mutant in hereditary cataracts. Biochemistry 39: 15791-15798, 2000

Srivastava S, Dixit BL, Ramana KV, Chandra A, Chandra D, Zacarias A, Petrash JM, Bhatnagar A, Srivastava SK: Structural and kinetic modifications of aldose reductase by S-nitrosothiols. Biochem J 358:111-118, 2001

Cobb BA and Petrash JM: a-Crystallin chaperone-like activity and membrane binding in age-related cataracts. Biochemistry 41:483-490, 2002

Cobb BA and Petrash JM: Factors influencing a-crystallin association with phospholipid vesicles. Molec Vision 8:85-93, 2002(http://www.molvis.org/molvis/v8/a12)

Wu X, Chen SG, Petrash JM, Monnier VM: Alteration of substrate selectivity through mutation of two arginine residues in the binding site of Amadoriase II from Aspergillus sp. Biochemistry 41: 4453-4458, 2002

Cecconi I, Scaloni A, Rastelli G, Moroni M, Vilardo PG, Costantino L, Cappiello M, Garland D, Carper D, Petrash JM, Del Corso, A, Mura U: Oxidative modification of aldose reductase induced by copper ion. Definition of the metal-protein interaction mechanism. J Biol Chem 277, 42017-42027, 2002

Chang Q, Harter TH, Griest TA, Murthy BSN, Petrash JM: Aldo-keto reductases in the stress response of the budding yeast Saccharomyces cerevisiae. In: The Emerging Role of Aldo-Keto Reductases in the Metabolism of Toxic Substances, (T. Penning, JM Petrash, eds.), pp. 225-238, American Chemical Society, Washington, DC, 2003

Petrash JM: All in the family: Aldose reductase and closely related aldo-keto reductases. Cell Molec Life Sci 61:737-749, 2004

Suryanarayana P, Kumar PA, Saraswat M, Petrash JM, Reddy GB: Inhibition of aldose reductase by tannoid principles of Emblica officinalis: Implications for the prevention of sugar cataract. Molec Vis 10, 148-154, 2004.

Hsu CD, Kymes S, Petrash JM: A transgenic model for human autosomal dominant cataract. Inv Ophthal Vis Sci 47:2036-2044, 2006 (Reprint)

Chang Q, Griest TA, Harter TM, Petrash JM: Phenotypic rescue of aldo-keto reductase null Saccharomyces cerevisiae by human aldose reductase. Biochem Biophys Acta (Molec Cell Res) Epub ahead of print: doi 10.1016/j.bbamcr.2006.10.009), 2006

Estey T, Wenston PA, Cantore M, Carpenter JF, Petrash JM, Vasiliou V: Mechanisms involved in the protection of UV-induced protein inactivation by the corneal crystallin ALDH3A1. J Biol Chem (Epub ahead of print: doi 10/1074/jbc.M607546200) 2006