Molecular Genetics Core Mission Statement

The Molecular Genetics Service Core assists investigators with routine molecular biology applications and all technologies associated with the production and maintenance of genetically altered mouse strains. By centralizing labor-intensive tasks and providing easy access to gene targeting at vastly reduced costs we remove the burden of setting up such technologies in individual laboratories. 

Eligibility

The services are available to all members of the Department of Ophthalmology and Visual Sciences, including adjunct faculty with primary appoints in other Departments. Anyone holding an NEI grant (regardless of departmental affiliation) is also eligible to use our services. 

 

Description of Services

Below is our list of current services

Meet the Molecular Genetics Service Core Team

Core Director

Questions and concerns should be addressed directly to Dr. Ferguson. 

Thomas Ferguson, PhD

Professor, Ophthalmology and Visual Sciences

Core Co-Director

Dr. Chen will assist Dr. Ferguson in managing the core, including reviewing projects, consultation, and addressing questions/concerns.

Shiming Chen, PhD

Professor, Ophthalmology and Visual Sciences

 

Director of the Molecular Genetics Core

 

Fee Schedule  – as of January 2018

Service

Cost

Transgenic, ES cell, CRISPR/Cas9

$ 750 per injection day

Rederivation

$ 250 per harvest day

Cryopreservation/Embryo storage

$ 200 per harvest day +Cost of liquid nitrogen

Sperm cryopreservation

$ 150.00 per harvest day +Cost of liquid nitrogen

IVF (in vitro fertilization)

$ 715

 ES cell services

Contact https://mescc.wustl.edu/ at the Washington University Mouse ES core; for services and pricing.

Fee Schedule  – As of July 2019

Service

Cost

Constructs CRISPR/Cas, transgenics, knockouts and other purposes 1,2

$ 175 per project to cover consumables

Oligonucleotides:

Billed at cost

Sequencing

$ 6 per reaction

Genotyping    

$ 2 per genotype

 Other (e.g. genotyping help) 

Negotiable

1 Oligo and/or sequencing are not included in the cost of a construct.

Transgene isolation is included in the cost of either standard or BAC transgenic constructs.