Molecular Genetics Core Mission Statement

The Molecular Genetics Service Core assists core investigators with routine molecular biology applications and all technologies associated with the production and maintenance of genetically altered mouse strains and recombinant DNA reagents. By centralizing labor-intensive tasks and providing easy access to gene engineering technologies at reduced costs, we remove the burden of setting up such technologies in individual laboratories.

Our services are provided at “cost” reducing the financial burden on already stressed grant budgets. For example, in our core, the production cost for generating a gene targeted mouse (from construct design to delivery of a founder mouse) cam be as little as $4000. Other Transgenic cores at Washington University typically range from $10-12,000. Commercial sources vary hugely in costs but may range from $30-60,000.

Eligibility

The services are available to all members of the Department of Ophthalmology and Visual Sciences, including adjunct faculty with primary appoints in other Departments. Anyone holding an NEI grant (regardless of departmental affiliation) is also eligible to use our services. 

Meet the Molecular Genetics Service Core Team

Core Director

Shiming Chen, PhD

Professor, Ophthalmology and Visual Sciences

  • Director of the Molecular Genetics Core

 

Dr. Chen will manage the core, including reviewing projects, progress and service costs.

Questions and concerns should be addressed directly to Dr. Chen. 

Thomas Ferguson, PhD

Professor, Ophthalmology and Visual Sciences

Dr. Ferguson will provide consultation in core management.

Susan Penrose

Core Technician

Susan provides expertise in the production of transgenic and knockout mice. Including management of production animals, pro-nuclear injection, blastocyst injection, embryo cytoplasm injection, embryo electroporation, cryopreservation/recovery of embryos and sperm, strain re-derivation, in vitro fertilization (IVF), genotyping of potential founders and advice in strain selection and founder screening.

Michael Casey

Core Technician

Mike provides support in many areas of molecular biology, including the design, production and validation of DNA constructs, siRNA, and CRISPR-Cas9/guide RNAs and genotyping of founder mice

Procedure for Core Usage

Projects are accepted from investigators on a first come-first served basis. Investigators are asked to fill out a simple, online job requisition form and typically send an email to Dr. Chen , describing the nature of the required services. This is followed by a discussion directly with the investigator (or their designee) to clarify the specifics of the project. Core investigators will be regularly informed about the progress of their project and are notified if problems persist or alternative strategies are required. If the project is straightforward and/or routine, the investigator deals directly with the core technicians.

If the cloning project is for the production of genetically altered mice, the project then moves to the microinjection/electroporation phase. Investigators are only placed in the queue for microinjection/electroporation when all necessary reagents (transgenes, ES cells, targeting contructs, single-guide RNAs) and a practicable screening strategy for founder mice are in hand. Appropriate mouse stains are then ordered and a sate is set for microinjection/electroporation and implatation. When mice are born the offspring are screened using the pre-established strategy (typically at 2-3 weeks of age). Following weaning positive mice are transferred to the investigators for further analysis.

Description of Services

Below is our list of current services

  1. We perform recombinant DNA cloning, including single vectors or oligo library constructions. We are available for consultation on any project related to molecular biology, transgenic mice (including BAC transgenic and targeted transgenic), knockout mice, CRISPR/Cas9 technology, etc.  For example:

a. Design and construction of constructs for transgenic mice (conventional or targeted transgenic), BAC transgenics, Knock-In/Knock-Out mice, and single-guide RNAs (sgRNA) for CrispR/Cas9-mediated genomic engineering.

b. Design and testing of genotyping PCR primers

c. Recombinant DNA cloning not related to transgenic mice (e.g. expression vectors, cDNA cloning, viral vectors and pooled constructs or libraries for high-throughput screens).  

Transgenic/Knockout

a. Production of Transgenic Mice: Transgenic animals are produced by injecting a DNA construct directly into the pronucleus of a newly fertilized mouse embryo or by electroporarted target DNA into one-cell embryos. Plasmid or BAC constructs can be used for microinjection. We use C57BL/6J inbred mice for these manipulations, however; we can accommodate other strains (e.g. F1 hybrids) by special request. 

We charge per injection/electroporation day and we typically do 2-4 injections.  The pups are screened for the presence of the transgene by a polymerase chain reaction genotyping assay. When the founder animals are at weaning age they are transferred to the investigator.   Transgenic founders are normally received seven to eight weeks after the original injections are performed when the mice are four to five weeks old.  

b.CRISPR/Cas-mediated Genomic Engineering: The use of embryonic stem (ES) cells has been the traditional method to produce gene targeting in mice, which is costly and time consuming, generally taking 9-12 months to generate single gene targeted offspring. This technology has been superseded by CRISPER/Cas-mediated genomic engineering where mice can be produced carrying multiple mutations, endogenous reporters, or conditional alleles. In this technology, specifically designed nucleotides are injected or electroporated directly into the one-cell embryo circumventing the need for selection of ES cells and chimera formation. Following target design, the generation of gene targeted mice cane be achieved in as little as 4 weeks. The molecular Genetics core has implemented all phases of this technology and is available to discuss individual needs. See Cell 157:1262, 2014 and Nature Protocols 9:1956, 2014 for more information.

c. Production of Chimeric Mice with ES Cells: The CRISPER/Cas technology produces limited success for a few applications, such as creation a conditional allele containing two loxP sites flanking a designated exon. The MG core maintains the option for ED cell-related services. Since Wash U no longer maintains an independent ES cell core, we will collaborate with the ES cell core in the University of Missouri. The UM ES cell core will perform ES cell transfection and clonal expansion after completing screens for desired targeting clones by our core. We will then generate chimeric mice by injecting the expanded ES cells into C57BL/6J blastocysts in our core animal procedure room (Rm 111A) located in SRF-W mouse facility. The resulting chimeric mice will be transferred to the investigator’s mouse facility 7-8 weeks after the original blastocyst injections. We charge ES services and per injection day, and we typically do 4 injections.

Dr. Chen will meet one-on-one with our investigators to explain the CRISPR/Cas technology and alternative approaches, and discuss how it might best be incorporated into their research strategies.

Embryo/Sperm Cyropreservation/Recovery

Freezing mouse embryos or sperm is a convenient way to preserve important mouse lines for possible future use while saving animal care per diems. Receiving mouse strains of interest from other institutions can be held in the LN2 freezer for subsequent resuscitation of the line by IVF.

-Sperm Cryopreservation is the preferred method for long term storage of mouse strains. The cost to freeze a line is minimal. Typically the method requires that 2 males of the strain of interest are sacrificed to harvest sperm for freezing. The cost is for each strain. Sperm will be maintained in liquid nitrogen and an annual storage fee will be assessed. 2 males, 10-20 weeks of age that are healthy and reproductively active are typically enough to guarantee recovery. Males are brought to 710C McMillan for sperm freezing.

-Embryo Cryopreservation is an alternative way of long-term preservation of mouse strains. Typically, seven to ten young male mice with your gene of interest will be needed to generate enough embryos for each freezing session. The males are transferred to SRF-W animal room 111B for mating. While the males are in room 111B animal care per diems will be billed directly to the PI. Wild type females, 3 weeks of age will be purchase by the PI and sent to room 111B. The delivery dates will be designated in a pre-planned schedule. The females are sacrificed and the embryos harvested and frozen down at the 8 cell stage. It may take several freezing sessions to freeze down enough embryos in the bank. The cost is for each session. A test thaw to blastocyst stage is done each session for each strain. Frozen embryos may also be send from other institutions for recovery in our facility. The fee is for the females used as recipients for the embryos. Frozen embryos will be maintained in liquid nitrogen and an annual storage fee will be assessed.

In vitro fertilization

Fresh sperm from 2 males or frozen sperm samples can be used for IVF. 20 wild type females are purchased and used for oocyte donors.

Other services available

Ovarian transplants, ovariectomy and vasectomies are offered and training can be provided for these services. Fees are determined at time of services

Consultation and Training

Consultation and training in PCR techniques and breeding strategies.

Rederivation

Mice that have been exposed to pathogens or transfers of “dirty” mice from other facilities can be made free of disease through the rederivation. The Division of Comparative Medicine (DCM) currently provides rederivation services for the infected mice. If you plan to import dirty mice from another institution, please consult Susan Penrose for the options and details about DCM rederivation services.

Molecular Genetics Service Core Fees (FY20)

Fee Schedule  – as of January 2020

Transgenic Services:

Microinjection/Electroporation:

Transgenic, CRISPR/Cas9, ES cells: $750 per injection day

Cryopreservation/Embryo storage: $250 per harvest day + cost of liquid nitrogen

Sperm Cryopreservation: $150 per harvest day + cost of liquid nitrogen

IVF (in vitro fertilization): $715.00

IVF with enhanced CARD IVF medium: $875.00

Recombinant DNA vector services:

One $170 fee will be charged per project – Fee covers reagents and consumables

Service

Description of Service

Standard Cloning

cloning a PCR insert into the vector of choice
Guide Test For CRISPER/Cas-mediated genomic engineering
Simple Donor Construct Design and production of a simple construct for CRISPR/Cas-mediated genomic engineering e.g. inserting a tag into a construct
Complex Donor ConstructDesign and production of a simple construct for CRISPR/Cas-mediated genomic engineering e.g. producing a conditional allele
Guide RNA preparationPreparation of guide RNA for injection in a CRISPR/Cas experiment
CAS9 mRNA preparation Preparation of CAS9 mRNA for injection in a CRISPR/Cas experiment
Standard Knockout Construct ProductionDesign and preparation of a standard knockout construct e.g. insertion LoxP sites
Conditional Knockout Construct ProductionDesign and preparation of a condition knockout construct e.g.
Bac TransgeneDesign and preparation of a BAC transgene

Extra pricing based on project need pass-through costs

Service Description of Service Cost
DNA sequencing

Preparation of sample for sequencing and the cost of the sequencing

$6 per reaction

Complex DNA sequencing

Preparation of sample for sequencing and the cost of the

sequencing for GC rich sequencing or other difficult to sequence DNA
$8 per reaction

Standard DNA Oligos

Ordered from IDT – $0.20 per base + $11.95 shipping if order is less than $50Variable

Speciality DNA Oligos    

Ordered if needed for projectVariable

Plasmid

Ordered if unique plasmid is needed for project

Variable
GenotypingPCR analysis of DNA/tissue sample$2.00 per sample
Midi PrepLarger scale preps$13.64 per prep
Mini PrepTo grow up small volume of construct$1.22 per prep

ES Cell Services:

ES cell culture, transfection and clonal expansion: This will be done in the Animal Modeling Core at the University of Missouri. We will negotiate the best price for these services. Our core will do ES cell screens:

Southern blots (e.g. ES cell screen, Mouse tails, etc.)
$500.00 – Standard enzymes
$400.00 – Expensive enzyme (Provided by the investigator)
$200.00 – ES cell Screen via PCR (cost per ES cell tranfection, including expanded clones).

Other Services (e.g. genotyping help) – Variable