Molecular Genetics Core Mission Statement

The Molecular Genetics Service Core assists investigators with routine molecular biology applications and all technologies associated with the production and maintenance of genetically altered mouse strains. By centralizing labor-intensive tasks and providing easy access to gene targeting at vastly reduced costs we remove the burden of setting up such technologies in individual laboratories. 

Eligibility

The services are available to all members of the Department of Ophthalmology and Visual Sciences, including adjunct faculty with primary appoints in other Departments. Anyone holding an NEI grant (regardless of departmental affiliation) is also eligible to use our services. 

 

Description of Services

Below is our list of current services

Meet the Molecular Genetics Service Core Team

Core Director

Questions and concerns should be addressed directly to Dr. Ferguson. 

Thomas Ferguson, PhD

Professor, Ophthalmology and Visual Sciences

Core Co-Director

Dr. Chen will assist Dr. Ferguson in managing the core, including reviewing projects, consultation, and addressing questions/concerns.

Shiming Chen, PhD

Professor, Ophthalmology and Visual Sciences

Fee Schedule  – as of January 2018

Service

Cost

1.    Transgenic Services

 

Transgenic, ES cell, CRISPR/Cas9

$750 per injection day

Rederivation

$250 per harvest day

Cryopreservation/Embryo storage

$200 per harvest day +Cost of liquid nitrogen

Sperm cryopreservation

$150.00 per harvest day +Cost of liquid nitrogen

IVF (in vitro fertilization)

$715

2.    Constructs for CRISPR/Cas-mediated genomic engineering 1,2

Guide testing

$300 per test

Donor construct simple (e.g. inserting Tag)

$250

Donor construct complex (e.g. conditional allele

$350

Guide RNA preparation for injection:

$50 per guide

Cas9 mRNA preparation for injection

$75 per project

Sequencing

$7 per reaction

Oligonucleotides:

Billed at cost

Specialty materials (e.g. obtaining a BAC clone):

Billed at cost

3.    Constructs for traditional transgenics, knockouts and other purposes: 1,2

Conditional knockout (e.g. insert loxP sites):  

$350

Standard Knockout (e.g. insert a neo cassette)

$250

BAC transgenic

$350

Standard Transgenic construct         

$175

Transgene isolation    

$75

Standard cloning

$175

Oligonucleotides:

Billed at cost

Sequencing

$7 per reaction

4.     Other (e.g. genotyping help)           

Negotiable

5.     ES cell services

Contact https://mescc.wustl.edu/ at the Washington University Mouse ES core; for services and pricing.

1 Oligo and/or sequencing are not included in the cost of a construct.

Transgene isolation is included in the cost of either standard or BAC transgenic constructs.