Molecular Genetics core

Mike provides support in many areas of molecular biology, including the design, production and validation of DNA constructs, siRNA, and CRISPR-Cas9/guide RNAs and genotyping of founder mice

Susan provides expertise in the production of transgenic and knockout mice. Including management of production animals, pro-nuclear injection, blastocyst injection, embryo cytoplasm injection, embryo electroporation, cryopreservation/recovery of embryos and sperm, strain re-derivation, in vitro fertilization (IVF), genotyping of potential founders and advice in strain selection and founder screening.